Overexpression stable cell lines

Stable Cell Line Development Service by OriGene

Generating custom cell lines is a tedious and complex process. OriGene offers a custom stable cell line development service that can be designed for knockdown, knock-in, or knockout of virtually any protein of interest (constitutive and inducible options available). Take advantage of their expertise in gene expression and highly effective gene delivery technologies to produce high-quality cell lines.

What they offer

• Over Expression (ORF)
  • Inducible
  • Constitutive
• Knockdown (shRNA)
  • Inducible
  • Constitutive
• Knock-in (CRISPR)
• Knockout (CRISPR)

Service Highlights

• Fast turnaround time: 2-3 months
• Choose from OriGene’s extensive ORF clone collection or provide your own expression cassette
• Rigorous and customizable QC validation process
• Professional, Global Technical Support

Cell Line Development Workflow

Inducible Stable Cell Line Development

Inducible expression is a valuable tool for functional analysis of assay systems with toxic targets. OriGene's All-in-one Tet-On system is a new and improved version of the original Tet-On systems, designed to significantly stimulate expression of the downstream gene of interest (GOI).

Custom Overexpression stable cell lines by System BioSciences

Reliable, high-quality custom stable cell line generation for overexpression of your protein- or regulatory RNA-of-interest

Leverage SBI’s viral and non-viral vectors in robust overexpression cell lines

Whether you want constitutive or inducible overexpression of a protein or regulatory RNA, SBI’s services team can create the construct and the cell line. They have access to a range of technologies—including stably integrating viral and non-viral vectors—and an expert team experienced at working with a variety of cell lines, including adherent and suspension cells. The result is hundreds of successfully constructed stable cell lines for highly satisfied customers.

Why choose SBI’s Stable Cell Line Services?

• Save time by having SBI build your custom cell lines
• Leverage our highly-regarded gene expression and delivery technologies
• Accomplish more with our fast turnaround times and end-to-end services
• Turn to the company that other companies turn to—we are a trusted stable cell line manufacturer for many large pharma/biotech companies
• Enjoy consistent quality, confidentiality, and on-time delivery with all projects completed on-site in our Palo Alto, CA, facility

Available gene expression and delivery technologies

Viral Systems

• Lentivectors—high titer and reliable
  • Random integration
  • Limited insert size
  • High copy number

• Lentivirus Packaging—reliable gene delivery
  • High and ultra-high titers
  • Efficient transduction
  • Optimized reagents

Non-viral Systems

• PhiC31 Integrase System—one-step single-copy integration
  • Site-specific integration
  • Unlimited insert size
  • Single copy number

• PinPoint Targeted Integration System—excellent for creating isogenic cell lines
  • Site-specific integration
  • Unlimited insert size
  • Single copy number

Contact us for more information