News - Sanbio

EV-GuardTM EV Storage Buffer

Written by Marketing Marketing | May 31, 2023 10:19:58 AM

V-GuardTM EV Storage Buffer (EV-GuardTM) is an innovative solution designed to safeguard extracellular vesicles (EVs) and maintain their biological activities during storage. With its advanced formulation, EV-GuardTM effectively preserves the integrity and stability of EVs under various storage conditions, including multiple freeze-thaw cycles. Whether stored at 4°C, -20°C, or -80°C, EV-GuardTM shields EVs from degradation and ensures their optimal performance.

  • Ease of Use - Simply resuspend EV pellets in the 1x EV-GuardTM buffer or add the 10X concentrated EV-GuardTM buffer directly to the EV fractions from size exclusion column, making it a quick and easy step to preserve your EVs.
  • Superior Protection – Achieve up to 98% EV conservation, significantly higher than with PBS, even after multiple freeze-thaw cycles, ensuring your EVs are well preserved and retained for your downstream applications.
  • Preserve Functionality – EVs have better transfection efficiency in EV-GuardTM than in PBS after multiple freeze-thaw cycles and long-term storage, assuring your EVs remain biologically active and ready to use.
Catalog number Description Size
EXSBA-1 EV-GuardTM EV Storage Buffer – 1X 40 mL
EXSBA-10 EV-GuardTM EV Storage Buffer – 10X concentrated 4 mL

Overview

The impact of EV-GuardTM EV storage buffer: Preventing EV degradation, preserving EV integrity, and maintaining functionality:
Extracellular vesicles play a crucial role in cell-to-cell communication, carrying various biomolecules such as proteins, lipids, and nucleic acids. They have gained significant interest in research and clinical applications, such as diagnostics, therapeutics, and drug delivery. Preservation of the physical and biological characteristics of EVs is crucial for their use in downstream applications, making storage a key consideration.

Typically, EVs are suspended in PBS and stored at -80°C for long-term storage, or at -20°C/4°C for short-term storage. However, these storage conditions may be insufficient to prevent damage to and fragmentation of EVs, which could alter their functional properties.

The EV-GuardTM EV Storage Buffer is specifically formulated to prevent EV degradation, ensuring their biological activities are well-maintained. This feature is critical to the success of downstream research and clinical endeavors. With EV-GuardTM, your precious EV samples are well-preserved and ready for use in various applications.

Invest in EV-GuardTM EV Storage Buffer today and protect your valuable EV samples for tomorrow’s discoveries.

How It Works

The buffer is designed in both 1X and 10X concentrated formats to suit the needs for customers who choose to use different EV isolation methods which result in concentrated EV pellets (e.g., by ultracentrifugation, ExoQuick or other precipitation-based methods) or EV suspensions (e.g., by SmartSEC or other size exclusion-based methods).

Using the EV-GuardTM EV storage buffer – 1X

  1. Resuspend purified EV pellet in 100 ul - 500 ul of 1X EV-GuardTM EV Storage Buffer.
  2. Optional: Perform a protein quantitation assay (e.g., BCA or Qubit assay) to determine the amount of protein in your sample.
  3. Aliquot EV samples into appropriate volumes for storage.

Using the EV-GuardTM EV storage buffer – 10X

  1. Add 10X concentrated EV-GuardTM EV Storage Buffer to purified EV suspension to a final concentration of 1X. Gently pipet to mix.
  2. Optional: Perform a protein quantitation assay (e.g., BCA or Qubit assay) to determine the amount of protein in your sample.
  3. Aliquot EV samples into appropriate volumes for storage.
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