Lentivirus is a powerful gene delivery tool. Lentivirus can transduce most cells, including easy-to-transfect cells, hard-to-transfect cells, dividing, non-dividing and primary cells. The transduction efficiency can easily reach to 100% for most common cell lines.
Lentiviral transfer vectors need essential viral genes to package into lentiviral particles. Lentiviral packaging genes are provided on separate plasmids – lentiviral packaging plasmids.
Virus titration will show how successful your lentivirus production is and will help to trouble shoot if viral transduction fails. Therefore virus titration is necessary. In addition, if your cells are hard to transduce and optimization is needed by using different MOI (Multiplicity Of Infection), the lentiviral titer needs to be measured.
If the lentiviral titer is low, the virus can be concentrated quickly with Lentiviral Concentrator, easily reaching to 10^9 TU/mL. If higher titer is needed or ultra pure virus is needed for in vivo animal studies, ultra centrifugation is needed.
Lentiviral particles are enveloped viruses and tend to lose infectivity during storage, even at -80°C. The solution is a lentiviral storage buffer, Lentivirus Stabilizer, which can preserve the infectivity for at least one year at - 80°C.
OriGene Technologies offers products to help you improve your lentivirus production. Four product highlights of OriGene:
In addition, the OriGene Lentiviral Titer Kit (to measure virus titer in 4 hours) and the OriGene Lentiviral Stabilizer (to preserve the infectivity for at least one year at -80ºC) can help to improve your Lentivirus Production.
Sanbio is ready to help you with your lentiviral production. Please contact support@sanbio.nl with your questions.