Sanbio would like to highlight two of their suppliers in relation to 3D Cell Culture. High-quality biotechnology companies MBL International and ScienCell Research Laboratories.
First we explain more about Afamin/Wnt3a CM of MBL International. Secondly you will find an overview of the 3D Cell Culture products of ScienCell.
MBL International: Afamin/Wnt3a CM
Organoid Culture
Wnt signaling is known to be involved in early development, maintenance and regeneration of stem cells, and in cancer formation. Wnt signaling has also been found to play an important role in the growth and maintenance of these processes. In particular, Wnt3a has been revealed to be an essential niche component for maintaining the proliferation of Lgr5-positive stem cells in intestinal epithelial cells and is used for the production of various digestive organoids such as the small intestine, large intestine, stomach, pancreas and liver. Although Wnt3a has been conventionally used for the culture of gut organoids, it is a fat-soluble protein, so it forms aggregates in serum-free medium and cannot exert its activity sufficiently. In 2016, Mihara et al. found that high Wnt3a activity can be maintained by forming a complex with Wnt3a by Afamin, which is one of the components of serum. In addition, by using Afamin and Wnt3a complex for organoid culture, long-term culture of organoid becomes possible. This new medium will result in optimal success for your organoid experiments.
| Article number | Description |
| J-ORMW301R | Afamin/Wnt3a CM |
Mechanism of Wnt3a Stabilization by Afamin Proteins
Afamin/Wnt3a CM increased Lgr5 Positive Stem Cells Expressed td Tomato
ScienCell: 3D Cell Culture portfolio
The California-based biotechnology company ScienCell is the largest supplier of normal primary cells. Within their large portfolio of products they also offer 3D Cell Culture products.
Key features of ScienCell's 3D Cell Culture Products
- Convenient: Ready-To-Use 3D spheroids are delivered frozen.
- Comprehensive: All-Inclusive 3D Cell Culture Kits contain everything you need for performing 3D cell culture.
- Easy-to-use: No prior experience in 3D cell culture is required.
- Functional: Cell-specific functional markers are maintained in 3D cell culture.
- Reproducible: 3D cell structures form in a robust and homogeneous manner.
3D Ready-To-Use Cell Culture Spheroids Kits
Unique features
- Innovative: ScienCell is the only company to offer Ready-To-Use spheroids.
- Ready-to-use: Obtains hundreds of uniform 3D spheroids per well in 24 hours after thawing.
- High-throughput: Just thaw and plate spheroids directly into 24-, 48-, or 96-well plates.
- Simple: No experience in 3D cell culture is required.
- Functional: Maintains the expression of functional markers.
Gain New Biological Insights with ScienCell’s Ready-to-use 3D Spheroids
An overview of the Ready-to-use 3D Cell Culture Spheroid Kits
| Article number | Label in above image | Description |
| SCCSP3D-8768 | Blood Brain Barrier Model | Ready-To-Use 3D Human Blood Brain Barrier Spheroid Kit with HBMEC |
| SCCSP3D-8738 | Blood Brain Barrier Model | Ready-to-use 3D Human Blood Brain Barrier Spheroid Kit |
| SCCSP3D-5000 | Liver Model | Ready-to-use 3D Hepatic Stellate-Endothelial Cell Spheroids |
| SCCSP3D-5300 | Liver Model | Ready-to-use 3D Hepatocyte-Stellate Cell Spheroids |
| SCCSP3D-8750 | Liver Model | Ready-to-use 3D Human Hepatic Stellate Cell Monoculture Spheroids |
| SCCSP3D-1520 | Cerebral Cortex Model | Ready-to-use 3D Human Cortical Spheroids |
| SCCSP3D-6540 | Ocular Model | Ready-To-Use 3D Human Retinal Pigment Epithelial Spheroids |
| SCCSP3D-3200 | Lung Model | Ready-to-use 3D Human Pulmonary Alveolar Epithelial Cell Spheroids |
| SCCSP3D-3210 | Lung Model | Ready-To-Use 3D Human Airway Spheroids |
| SP3D-4100 | Kidney Model | Contact support@sanbio.nl for more information. |
| SP3D-4108 | Kidney Model | Contact support@sanbio.nl for more information. |
| SP3D-7500 | Mesenchymal Stem Cell Model | Contact support@sanbio.nl for more information. |
| SCCSP3D-4650 | Cartilage Model | Ready-to-use 3D Human Chondrocyte-articular Spheroid Kit |
| SCCSP3D-8748 | Bone Model | Ready-to-use 3D Osteogenesis-Angiogenesis Coupling Kit |
| SCCSP3D-4600 | Bone Model | Ready-to-use 3D Human Calvarial Osteoblast Spheroid Kit |
All-Inclusive 3D Cell Culture Spheroid Kits
Key features
- Convenient: Easy-to-use comprehensive kits.
- Flexible: Allows manipulations of specific variables using the All-Inclusive 3D Cell Culture Spheroid Kits.
- Uniform: Hundreds of 3D spheroids develop uniformly in each well using specially-formulated 3D cell culture medium.
- Reproducible: Obtains a statistically significant sample size from hundreds of 3D spheroids per well, enhancing the accuracy of your experimental data.
Homogenous Formation of 3D Primary Spheroids
An overview of the All-Inclusive 3D Cell Culture Spheroid Kits
| Article number | Label in above image | Article description |
| SCC3D-8738 | Blood Brain Barrier Model | All-inclusive 3D Human Blood Brain Barrier Spheroid Formation Kit |
| SCC3D-R8738 | Blood Brain Barrier Model | All-inclusive 3D Rat Blood Brain Barrier Spheroid Formation Kit |
| SCC3D-5000 | Liver Model | All-Inclusive 3D Human Hepatic Stellate-Endothelial Cell Spheroid Formation Kit |
| SCC3D-8750 | Liver Model | All-inclusive 3D Human Hepatic Stellate Cell Monoculture Spheroid Formation Kit |
| SCC3D-1520 | Cerebral Cortex Model | All-Inclusive 3D Human Cortical Spheroid Formation Kit |
| SCC3D-6540 | Ocular Model | All-inclusive 3D Human Retinal Pigment Epithelial Spheroid Formation Kit |
| SCC3D-4650 | Cartilage Model | All-inclusive 3D Human Chondrocyte-articular Spheroid Formation Kit |
| SCC3D-8748 | Bone Model | All-inclusive 3D Osteogenesis-Angiogenesis Coupling Kit |
| SCC3D-4600 | Bone Model | All-inclusive 3D Human Calvarial Osteoblast Spheroid Formation Kit |
Collagen I-Based 3D Cell Culture Kits
Main Features
- Convenient: Easy-to-use comprehensive kits.
- Relevant: Utilizes extracellular matrix to mimic the in vivo
- Reproducible: Achieves reliable 3D experimental results using highly quality primary cells and reagents from ScienCell.
- Functional: Displays cell-specific functional markers in ScienCell’s 3D collagen-based models.
3D Blood Brain Barrier (BBB) Model
Overview of the Collagen-Based 3D Cell Culture Kits
| Article number | Label in above image | Article description |
| SCC8738 | Blood Brain Barrier Model | Human Blood Brain Barrier Modeling Kit |
| SCC8728 | Blood Brain Barrier Model | 3D Endothelial–Pericyte Coculturing Kit |
| Kidney Model | ||
| SCC8708 | Vascular Biology Model | 3D Embedded Tubule Formation Kit |
| SCC8718 | Vascular Biology Model | 3D Network Formation Assay Kit |
3D Cell Culture Related Products
To complement the wide-range of 3D cell culture kits, ScienCell also offers 3D cell culture related products. ScienCell strives to provide you with reliable cell culture products and technical support to help you ease transition from 2D to 3D culture models.
| Article number | Article description |
| SCC0353 | Ultra-Low Binding Culture Plate (96-well) |
| SCC0343 | Ultra-Low Binding Culture Plate (48-well) |
| SCC0383 | Ultra-Low Binding Culture Plate (24-well) |
| SCC0373 | Ultra-Low Binding Culture Plate (6-well) |
| SCC8688 | 3D Collagen I Matrix Kit |
| SCC8178 | Collagen I-3D Gelling Kit |
| SCC8808 | 3D Gel Staining Prep Kit |
| SCC8158 | In Vitro Tube Formation Assay |
| SCC8698 | 3D Basic Embedded Tubule Formation Kit |
